Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Expression of CD47 in epithelial ovarian cancer tissues (100×) according to IHC staining. Specimens were stained with anti-CD47 polyclonal antibody. CD47 exhibits a predominantly membranous staining pattern, with no nuclear staining observed. A scale of 0–3 was employed: 0: ≤25% staining, 1: >25–50% positivity, 2: >50–75% positivity, cells showed membranous and/or cytoplasmic expression, 3: intense membranous and/or cytoplasmic expression of at least 75% of the tissue section. (A) CD47 IHC staining score of 0; (B) CD47 IHC staining score of 1; (C) CD47 IHC staining score of 2; (D) CD47 IHC staining score of 3. IHC, immunohistochemical.

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing, Immunohistochemistry, Staining, Immunohistochemical staining

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Patient characteristics by pre-NACT CD47 expression

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques:

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Expression of CD47 in epithelial ovarian cancer tissues

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing, Immunohistochemistry

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Change of CD47 expression pre- and post-NACT. NACT, neoadjuvant chemotherapy.

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Wilcoxon signed ranks test of the CD47 expression pre- and post-NACT

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Kaplan-Meier curves of CD47 expression for OS and PFS. The figure shows the Kaplan-Meier curves of OS and PFS for CD47 high and low expression in the cohort of 74 patients with EOC who received neoadjuvant chemotherapy, and four cases of stage I and II ovarian cancer were excluded from the analysis. EOC, epithelial ovarian cancer; OS, overall survival; PFS, progression-free survival; NACT, neoadjuvant chemotherapy.

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Multivariate prognostic analysis

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: The expression of CD47 mRNA in ovarian epithelial cells (HOSE) and ovarian cancer cells (SKOV3 and ES2) according to RT-qPCR analysis of CD47 mRNA levels. The levels of CD47 mRNA expression in ES2 and SKOV3 cells were found to be significantly higher than those in HOSE cells (P<0.001). ***, P<0.001. RT-qPCR, reverse transcription quantitative polymerase chain reaction; mRNA, messenger RNA; HOSE, human ovarian surface epithelial cell.

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: The CD47 mRNA expression in SKOV3 and ES2 cells both with and without cisplatin treatment. A control group was treated with 0 µM of cisplatin, while a second group was treated with 4 µM of cisplatin for 48 hours to assess the expression of CD47 mRNA. (A) The expression of CD47 mRNA in SKOV3 treated with 0 and 4 µM cisplatin for 48 hours. Following 48 hours of treatment with 4 µM of cisplatin, a notable elevation in CD47 mRNA was observed (P<0.001). (B) The expression of CD47 mRNA in ES2 treated with 0 and 4 µM of cisplatin for 48 hours. Following a 48-hour treatment with 4 µM of cisplatin, a notable elevation in CD47 mRNA was evident (P<0.001). ***, P<0.001. mRNA, messenger RNA; DDP, cisplatin.

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing, Control

Journal: Gland Surgery

Article Title: Chemotherapy-induced increase in CD47 expression in epithelial ovarian cancer

doi: 10.21037/gs-24-400

Figure Lengend Snippet: Expression of cell surface CD47 protein in SKOV3 and ES2 cells following cisplatin treatment. (A) Following a 48-hour cisplatin treatment period, the mean fluorescence intensity of the surface CD47 protein in SKOV3 cells demonstrated a statistically significant increase (P<0.001). (B) Following a 48-hour cisplatin treatment period, the mean fluorescence intensity of the surface CD47 protein in ES2 cells demonstrated a notable increase (P<0.001).

Article Snippet: Anti-CD47 polyclonal antibody (cat. no. AF4670; R&D Systems, Minneapolis, MN, USA) was added at a dilution of 1:150 to each section and incubated overnight at 4 °C ( ).

Techniques: Expressing, Fluorescence

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Representative example of CD47 expression in soft tissue sarcomas. A leiomyosarcoma 0; no staining of tumor cells, B undifferentiated pleiomorphic sarcoma 1 + ; less than 1/3 of the total tumor area positive (focal positivity), ( C ) dedifferentiated liposarcoma 2 + ; 1/3 – 2/3 of the total tumor area positive (moderate positivity) and D myxofibrosarcoma 3 + ; more than 2/3 of the total tumor area positive (diffuse positivity). Image magnification was 40 × and scale bars indicate 50 μm

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing, Staining

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Expression of CD47 molecule among various histological subtypes of soft tissue sarcoma (STS). A CD47 scale positivity in ( n = 55) and B frequency of CD47 scale positivity among grade (G)1 + G2 and G3. C CD47 scale positivity and D frequency in liposarcoma (LPS), myxofibrosarcoma (MFS) and undifferentiated pleiomorphic sarcoma (UPS) ( n = 47). E CD47 scale positivity ( n = 22) and F its frequency among G1 + G2 and G3 in LPS. G CD47 scale positivity in LPS divided according to histological subtypes. H frequency of CD47 scale positivity in myxofibrosarcoma (MFS) ( n = 11) and I its frequency between G1 + G2 and G3. J CD47 scale positivity in high-grade undifferentiated pleiomorphic sarcoma (UPS) ( n = 16). CD47 scale positivity indicators: 0 no staining of tumor cells, 1 + expression in less than 1/3 of tumor tissue, 2 + expression in 1/3 to 2/3 of tumor tissue, and 3 + expression in more than 1/3 of tumor tissue. p values were calculated using Mann–Whitney U test

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing, Staining, MANN-WHITNEY

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Chi-square test of independence to assess the association between CD47 expression and grade, metastases, and death

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing, Sampling

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Chi-square test of independence to assess the association between CD47 expression (CD47 3 + vs CD47 0–2 +) and grade, metastases, and death

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing, Sampling

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Association between CD47 expression and clinical outcome of patients with soft tissue sarcoma (STS). Kaplan–Meier curves depict the association between CD47 and A overall survival (OS), B metastasis-free survival, and C progression-free survival (PFS) in patients divided according to CD47 scale positivity. Kaplan–Meier curves show D OS, E metastasis-free survival, and PFS in CD47 0–2 + and CD47 3 + patients. Censored patients have not experienced the event of interest (progression, metastasis, death) by the end of the study period. The small vertical lines serve as a visual cue to underscore the presence of censored observations. p values were calculated by log-rank test

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Cox proportional hazards model assessing the prognostic significance of CD47 expression on overall survival (OS)

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: A comprehensive analysis of CD47 expression in various histological subtypes of soft tissue sarcoma: exploring novel opportunities for macrophage-directed treatments

doi: 10.1007/s00432-024-05661-1

Figure Lengend Snippet: Cox proportional hazards model assessing the prognostic significance of CD47 expression on progression-free survival (PFS)

Article Snippet: Next, the tissue slides were stained with a polyclonal anti-CD47 antibody (PA5-80435, Thermo Fisher Scientific, Massachusetts, USA).

Techniques: Expressing

Journal: Genome Biology

Article Title: In vivo perturb-seq of cancer and microenvironment cells dissects oncologic drivers and radiotherapy responses in glioblastoma

doi: 10.1186/s13059-024-03404-6

Figure Lengend Snippet: Ptprc loss attenuates macrophage phagocytosis of malignant cells. A Schematic of time lapse microscopy assays for co-cultured J774 macrophages ( Ptprc knockdown or control) and Ramos lymphoma cells. pHrodo red intracellular indicator dye is used to measure extent of phagocytosis following opsonization of malignant cells with α-CD47 and α-CD20 antibodies. B Distribution of mean Ptprc (CD45) expression measured by flow cytometry. n = 3 replicates. C Phagocytosis time lapse microscopy assay for uptake of Ramos cancer cells by J774 macrophages. p values, two-way ANOVA comparing each Ptprc perturbation to both negative control sgRNAs. n = 3 cell culture wells. C Images of J774 macrophages with indicated genotypes co-cultured with pHrodo red-labelled malignant Ramos cells after 4 h incubation. Scale bar, 100 μm. Representative of two independent experiments performed in biological triplicate wells

Article Snippet: Anti-human CD20 (BioXCell, #SIM0008, lot no. 909923J2) and anti-human CD47 (BioXCell, #BE0019-1, lot no 878622D1) were then both added to final concentrations of 500 ng/mL.

Techniques: Time-lapse Microscopy, Cell Culture, Knockdown, Control, Expressing, Flow Cytometry, Negative Control, Incubation